THE INFILTRATION ABILITY OF BACILLUS SUBTILIS CELLS INTO XYLEM VESSELS OF CUT ROSE CV. 'SONIA': CRYO-SEM OBSERVATIONS

H. Put, W. Klop, A.C.M. Clerkx
Cryo-SEM (Scanning Electron Microscopic) observations have been made of transverse and longitudinal cross-sections of xylem vessels of cut flowers of Rosa cv. 'Sonia'. The flowers were collected after a vase life of 48 hr and four different vase waters were used for this incubation period: sterilized deionized water (1), to which was added: (2) Al2(SO4)3. solution; (3) Bacillus subtilis cells; (4) Al2(SO4)3 and Bacillus subtilis cells. Electron micrographs of stem sections were taken in de Jeol 35C scanning electron microscope at 15 kV acceleration voltage. For X-ray microanalysis the Edax 9900 system was used, and the stem specimens were coated with carbon. The results show: (1) Cryo-SEM micrograph images of Rosa stem specimens showed more relevant structural details compared with those retained in SEM specimens fixed with gluteraldehyde; (2) cryo-SEM images confirm previously reported observations by the same authors showing that the cut surface of Rosa stems can act as a coarse-threaded filter. Only a small fraction of Bacillus cells entered with the sapstream into the vascular system. The remaining fraction was attached to the cut surface or adhered on the lower part of the vessels; (3) when an aluminium sulphate solution and Bacillus cells were added to the vase fluid, the cryo-SEM images showed that the bacterial
Put, H., Klop, W. and Clerkx, A.C.M. (1991). THE INFILTRATION ABILITY OF BACILLUS SUBTILIS CELLS INTO XYLEM VESSELS OF CUT ROSE CV. 'SONIA': CRYO-SEM OBSERVATIONS. Acta Hortic. 298, 303-312
DOI: 10.17660/ActaHortic.1991.298.37
https://doi.org/10.17660/ActaHortic.1991.298.37
English

Acta Horticulturae