CHARACTERISATION OF ISOLATES OF FUSARIUM OXYSPORUM FROM CARNATIONS IN VICTORIA.
Isolates of F. oxysporum, collected during a large scale survey of specialist carnation propogators in Victoria, were classified by cultural morphology, pathogenicity, vegetative compatibility, production of volatiles and a random amplified polymorphic DNA (RAPD) assay. Morphological and cultural characteristics did not correlate with the other techniques used, and were not consistent within groups. Of 81 isolates recovered from symptomless cuttings, pathogenicity testing to date has identified 25 to be pathogenic. Pairing of nitrate non-utilising mutants was used to assess vegetative compatibility of the isolates. Nineteen vegetative compatibility groups (VCG) were identified with 2 of the larger VCGs containing pathogenic isolates, while the remaining groups were non-pathogenic. Gás-chromatographic analysis of volatiles resulted in a distinctive chromatogram for representative isolates from one of the pathogenic groups. However, this method does not distinguish between the other pathogenic group and the non-pathogenic groups. The RAPD assay identified banding pattern differences in DNA samples from 4 of the VCG's identified, with one distinctive band common to the two pathogenic VCG's.
Kalc Wright, G., Pascoe, I., van Heeswijck, R., Guest, D. and Wimalajcewa, S. (1992). CHARACTERISATION OF ISOLATES OF FUSARIUM OXYSPORUM FROM CARNATIONS IN VICTORIA.. Acta Hortic. 307, 65-72