SOLUBLE PROTEIN CONTENTS, ISOZYME CHARACTERIZATION, BENOMYL RESPONSE, VEGETATIVE COMPATIBILITY AND MYCELIAL GROWTH OF FUSARIUM OXYSPORUM F. SP. DIANTHI POPULATIONS
Fusarium oxysporum Schlecht f. sp. dianthi (Prill. and Del.) Snyd. and Hans. is the most important soil pathogen affecting carnation plantations in the Bogotá Plateau. Introduced in imported cuttings, it has colonized many flower growing areas. Research from Italy, Holland and the United States has shown the existence of different races of the pathogen. This present study was started in 1988 in order to determine the characteristics of the pathogen such as its taxonomic diversity, physiology, genetics and biochemistry. 100 isolates of the pathogen collected from different areas in the Bogotá Plateau and 14 foreign isolates of races 1, 2, 4 and 8, were studied. The content of total soluble proteins as well as the presence of arylesterase enzyme polymorphism in these isolates were evaluated by electrophoresis on polyacrylamide gels. Differences between isolates were found for both of the aspects studied. Mutants were obtained from the isolates of the pathogen that do not utilize nitrates. These mutants permitted the application of vegetative compatibility tests as well as the determination of genetic relationships among pathogen races. In vitro responses to benomyl and mycelial growth rates showed heterogenity among isolates and helped to determine groups with similar reactions. The results of this study could prove to be important in the identification and characterization of the races of the pathogen.
Garcés de G, E., Orozco de A, M., Sinisterra, X., Medina, G., Acosta, O., Peñaranda, J. and Arbelaez, G. (1992). SOLUBLE PROTEIN CONTENTS, ISOZYME CHARACTERIZATION, BENOMYL RESPONSE, VEGETATIVE COMPATIBILITY AND MYCELIAL GROWTH OF FUSARIUM OXYSPORUM F. SP. DIANTHI POPULATIONS. Acta Hortic. 307, 73-82