PLANT REGENERATION FROM TISSUE CULTURED-ROOT IN ALLIUM VICTORIALIS L. SSP. PLATYPHYLLUM HULT.

Allium victorialis L. ssp. platyphyllum Hult. grows very slowly and it is necessary to establish the high efficient propagating method for its cultivation. In the present presentation, plant regeneration from in vitro cultured root was examined for multi-propagation.

Seedling were aseptically grown on Murashige & Skoog (MS) solidified medium containing 20 g/liter sucrose, 6-benzylaminopurine (BA) and -naphthaleneacetic acid (NAA). After 6 months of incubation, root tops enlarged on the media supplemented with 1.0 to 10 μM BA. Then, nodular calli were induced at the enlarged portion in the media supplemented with 0 to 1.0 μM NAA and 10 μM BA, and green spots and adventitious buds were differentiated on those calli. By transferring of adventitious buds to the medium without plant growth regulator, whole plants regenerated, especially, root growth was accelerated.

In those morphogenesis, the mass of vigorous dividing cells were generated from vascular cylinder on media containing high concentration of BA. Subsequently, adventitious buds were originated from the such mass.

It is assumed that cytokinin takes part in the adventitious bud formation on the cultured root. Plant regeneration from the root occurs growing underground a little in natural condition and a large number of transplant production is expected to conduct systematically by utilizing root-culture.