BIOTECHNOLOGICAL BREEDING OF ORNAMENTAL HONEYSUCKLES (LONICERA, CAPRIFOLIACEAE)
For centuries, breeders could only rely on sexual crossing to bring about genetic variability into plants. Today, with the advent of biotechnology, novel means to achieve this are available. The genus Lonicera includes climbing and shrubby species, widely cultivated in Europe, each with interesting traits, but which might be difficult to combine due to incompatibility problems. The use of biotechnology was therefore seen as a breeding alternative. Thus, stem, leaf and root callus cultures were induced for 4 climbing (L. periclymenum 'Serotina', L. x brownii 'Dropmore Scarlet', L. x heckrottii 'Gold Flame', L. japonica 'Hall's Prolific') and 2 shrubby (L. fragrantissima, L. nitida 'Maigrün') species, coupled with the establishment of cell suspensions from the stem and leaf calli of L. fragrantissima. All of these were used for assessments of organogenic competence. Plants have already been successfully regenerated from the leaf and stem calli of L. japonica 'Hall's Prolific' and from root calli of L. nitida 'Maigrün'. In addition, protoplasts were isolated from leaf and callus tissues and from cell suspension cultures of both climbing (L. periclymenum 'Serotina', L. x brownii 'Dropmore Scarlet') and shrubby (L. fragrantissima, L. nitida 'Maigrün') genotypes. Following culture, multicellular colonies were obtained (for all sources/genotypes), while complete protoplast-derived plants were regenerated from leaf protoplasts of L. nitida 'Maigrün'. These results open up the way for the exploitation of soma/protoclonal variation and somatic hybridization technologies as novel tools for honeysuckle breeding.
GEORGES, D., DECOURTYE, L. and OCHATT, S.J. (1992). BIOTECHNOLOGICAL BREEDING OF ORNAMENTAL HONEYSUCKLES (LONICERA, CAPRIFOLIACEAE). Acta Hortic. 320, 127-132