RAPID IN VITRO MULTIPLICALION OF VITIS VINIFERA L. THROUGH SHOOT TIPS AND NODAL SEGMENTS.

A. Singh, B. Sharma, R. Pandey
Multiple shoots were proliferated from shoot tips of grape cultivar Perlette. Shoot tips were collected from mature vines grown under partial shade. Explants were cultured in Murashige and Skoog basal medium (MS) supplemented with 5, 10, 15 and 20 uM 6-benzylaminopurine (BA). MS medium supplemented with 4% sucrose and 10 uM 6-benzylaminopurine resulted in a maximum rate of shoot multiplication (up to 18 folds) in terms of the number of nodes (propagule) formed per shoot. Higher concentrations of BA (15 and 20 uM) strongly inhibited shoot elongation.

Shoots (8 to 12 cm long) with 6 to 8 nodes produced in vitro were carefully cut below the node and inserted vertically in to the MS medium supplemented with 1, 5, 10, 15, and 20 uM indole-3-butyric acid (IBA). Primary root formation, its growth and branching was maximum with IBA level of 10 uM. All the cultures were grown at a temperature of 24 ± 2°C and illuminated for 16 hr. per day at 3,500 lux. Rooted shoots were transferred to the potting mixtures of soil, farm yard manure and sphagnum peat (1:1:1) and acclimatized. After hardening, plants were transplanted in the field. No phenotypic variation was observed during their first year of growth in the field.

Singh, A., Sharma, B. and Pandey, R. (1992). RAPID IN VITRO MULTIPLICALION OF VITIS VINIFERA L. THROUGH SHOOT TIPS AND NODAL SEGMENTS.. Acta Hortic. 321, 601-605
DOI: 10.17660/ActaHortic.1992.321.70
https://doi.org/10.17660/ActaHortic.1992.321.70

Acta Horticulturae