THE COURSE OF BEAN YELLOW MOSAIC VIRUS ELIMINATION FROM GLADIOLUS BY MERISTEM TIP CULTURE

H. Lilien-Kipnis, A. Stein, S. Levy
Bean yellow mosaic virus (BYMV) and cucumber mosaic virus (CMV) are the most prevalent viruses of Gladiolus. There is interest in production of virus-free propagating stocks of miniature hybrids recently bred in Israel. In this study, the role of meristem-tip size on percentage of BYMV elimination was examined. Also, the course of BYMV detectibility was followed by ELISA in hybrid cv. 'Yamit'. Meristems or larger tips were excised and cultured as single plantlets or as multiplying clusters from which families of plantlets originating from a single meristem or tip were subcultured. Plantlets from larger tips were found 90% infected by the third BYMV test, whereas, meristem-derived ones were only 34.5% infected by the fifth test and also one year later ex vitro. Both infected and seemingly uninfected plantlets were found to originate from the same meristems. It is suggested that more sensitive tests such as polymerase chain reaction (PCR) techniques would be useful in early detection of all infected plants which would significantly reduce the cost of preparing virus-free propagating stocks of Gladiolus.
Lilien-Kipnis, H., Stein, A. and Levy, S. (1992). THE COURSE OF BEAN YELLOW MOSAIC VIRUS ELIMINATION FROM GLADIOLUS BY MERISTEM TIP CULTURE. Acta Hortic. 325, 709-714
DOI: 10.17660/ActaHortic.1992.325.102
https://doi.org/10.17660/ActaHortic.1992.325.102

Acta Horticulturae