TISSUE CULTURE OF FLOWER-BULB CROPS: THEORY AND PRACTICE
During the last decades, tissue culture has evolved as a powerful tool for breeders and propagators. The literature describes many techniques that have been developed for the production of plants from various tissues. These plants may originate from explants, meristems, gametes, protoplasts, fused cells or transformed cells, thereby providing the basis for the development of methods to accelerate clonal propagation or to increase variation within a crop. Many of these techniques have also been described for flower-bulb crops. However, only a few have found their way to practical application. There are a number of reasons to explain this discrepancy. Technology transfer needs time. The literature often is very fragmentary, even on a subject as micropropagation. In most cases the technology costs too much. In some cases the potentialities of the technique are ignored and in others the techniques are too complicated. This paper reviews some of these features for propagation of flower-bulb crops by tissue culture.
van der Linde, P.C.G. (1992). TISSUE CULTURE OF FLOWER-BULB CROPS: THEORY AND PRACTICE. Acta Hortic. 325, 419-428