IN VITRO POLLINATION AND OVARY CULTURE AS A BREEDING TOOL IN WIDE HYBRIDIZATION OF LILIUM AND NERINE

J.M. van Tuyl, M.G.M. van Creij, M.P. van Dién
Using lily (Lilium L.) as a modelcrop, an in vitro pollination, fertilization embryo rescue system has been developed. By combining pollination techniques to overcome pre-fertilization barriers with in vitro methods to overcome post-fertilization barriers, wide interspecific lily crosses could be made more efficiently. In vitro cut-style pollination and in vltro grafted style techniques were successfully applied in various inter.-specific crosses. To overcome post-fertilization barriers, methods for ovary culture, ovary-slice culture and ovule culture were generated. Five Lilium species were used L. longiflorum, L. henryi, L. concolor, L. dauricum, L. candidum. The application of these methods resulted in many new hybrids between these species. To overcome intergeneric crossing barriers between the genera Nerine, Amaryllis and Lycoris in vitro pollination angi ovary culture techniques have been developed analogous to lily. Results are promising.
van Tuyl, J.M., van Creij, M.G.M. and van Dién, M.P. (1992). IN VITRO POLLINATION AND OVARY CULTURE AS A BREEDING TOOL IN WIDE HYBRIDIZATION OF LILIUM AND NERINE. Acta Hortic. 325, 461-466
DOI: 10.17660/ActaHortic.1992.325.63
https://doi.org/10.17660/ActaHortic.1992.325.63
English

Acta Horticulturae