GROWTH AND MINERAL UPTAKE BY EXPLANTS OF HYACINTHUS ORIENTALIS L. UNDER DIFFERENT TEMPERATURE TREATMENT
Cultures of adventitious shoots of hyacinths were maintained at 23°C or 4°C as well as chilled for various periods at 4°C and then transferred into 23°C. At each sampling period (every 2 weeks for 20 weeks) the changes in mineral composition of the medium and within the tissue of explants were analyzed. The cultures proved a gradation of morphological response depending on the temperature and longevity of the treatment. If continuously cultured at the same temperature the adventitious shoots did not form bulblets within 20 weeks of 4°C culture or within 10 weeks of 23°C. The development of bulblet occurred only after the shoots which were given low temperature treatment were transferred to 23°C. The highest levels of P, K and Mg in the shoots were observed in the period of sprouting of new leaves. In contrast with bulblets, the shoots showed their lowest content of these nutriens during the bulblet formation time. There was a rapid decrease of the pH of the medium after autoclaving and through 2nd week of culture of hyacinthus incubated at 23°C. The mineral analysis of the medium showed a different uptake of NH4 and NO3.
Bach, A., Cecot, A. and Gaweda, M. (1992). GROWTH AND MINERAL UPTAKE BY EXPLANTS OF HYACINTHUS ORIENTALIS L. UNDER DIFFERENT TEMPERATURE TREATMENT. Acta Hortic. 325, 481-486