UTILIZATION OF SOLUBLE CARBOHYDRATES IN SHOOT AND BULB REGENERATION OF HYACINTHUS ORIENTALIS L. IN VITRO
Changes in soluble sugar contents in a culture medium and in the tissue of hyacinth explants were noted. Cultured leaf explants were isolated from "chilled" shoots (at 4°C for 8 weeks) and another incubated at 23°C. Explants were cultured onto MS medium containing 6-benzyloaminopurine, a-naphtaleneacetic acid and/or paclobutrazol as well as varying sugar levels (3% and 6% of sucrose, glucose and fructose). Explants taken from "chilled" shoots and from "unchilled" but cultured onto medium with paclobutrazol formed bulblets, whereas explant from shoots incubated at constant 23°C produced a large amount of adventitious one-leaf shoots. About 10 percent of the sugar in the agar medium without paclobutrazol was hydrolyzed during autoclaving. In presence of the hyacinth explants exposed to low temperature the initial amount of soluble sugars in medium decreased to 15.9% (glucose), 26.9% (sucrose) and 45.2% (fructose) at the end of the culture period (12 weeks). Concentration and a variety of sugar affected shoot and bulblet regeneration. Glucose- and sucrose-containing medium was superior to fructose-containing medium in induction of shoots and bulblets. Concentration of soluble sugars increased considerably in the "chilled" explants as well as in the explants cultured on media with paclobutrazol, but was not parallel with its decreasing in the medium.
Bach, A., Pawlowska, B. and Pulczynska, K. (1992). UTILIZATION OF SOLUBLE CARBOHYDRATES IN SHOOT AND BULB REGENERATION OF HYACINTHUS ORIENTALIS L. IN VITRO. Acta Hortic. 325, 487-492