R.W. van den Bulk, H.P.J. de Vries-van Hulten, J.J.M. Dons
The regeneration of (doubled) haploid plants from isolated microspores offers a promising system for plant breeders. Lily microspores were isolated and cultured in order to study the induction of sporophytic development. The developmental stage of the microspores, an important factor in microspore culture, was shown to be positively correlated with flower bud length, facilitating the isolation of microspores of a specific stage. The viability of microspores of the Asiatic hybrid 'Whilito' after isolation was 70 to 90%, similar to the viability in vivo. During the first week of culture the viability decreased to approximately 25%. After four days of culture microspores were observed which had undergone additional nuclear divisions, resulting in multinucleate microspores containing up to eight nuclei. So far, however, further divisions did not take place. The formation of multinucleated microspores presumably is an indication of sporophytic development.
van den Bulk, R.W., de Vries-van Hulten, H.P.J. and Dons, J.J.M. (1992). FORMATION OF MULTINUCLEATE LILY MICROSPORES IN CULTURE. Acta Hortic. 325, 649-654
DOI: 10.17660/ActaHortic.1992.325.92
androgenesis, haploids, Lilium

Acta Horticulturae