PECTIC ZYMOGRAM ANALYSIS FOR CHARACTERIZING GENETIC DIVERSITY OF THE MANGO ANTHRACNOSE PATHOGEN.
Sixty-three isolates of the mango anthracnose pathogen Colletotrichum gloeosporioides, from naturally infected leaves, panicles, flowers, and immature and mature fruits were subjected to pectic zymogram analysis. The fungi were cultured in a minimal medium containing pectin to induce pectinase production. Culture filtrates containing extracellular pectinases were subjected to pectinpolyacrylamide gel electrophoresis and comparative analysis of the resultant zymograms lead to identification of 10 representative pectic zymogram groups (PZG). Two of the 4 PZGs produced by isolates from mature fruits were most prevalent, and one of these PZGs associated with mature fruits was also found to occur on immature fruits. The other prevalent PZG on mature fruits was also prevalent among leaf isolates. Pectic zymogram analysis not only revealed genetic diversity within the population of the mango anthracnose pathogen, but also indicated that isolates of various PZGs were differentially distributed on different tissues of mango trees. The technique is simple, rapid, and amenable to analysis of a large number of strains.
Gantotti, B.V. and Davis, M.J. (1993). PECTIC ZYMOGRAM ANALYSIS FOR CHARACTERIZING GENETIC DIVERSITY OF THE MANGO ANTHRACNOSE PATHOGEN.. Acta Hortic. 341, 353-359
Mangifera indica, Colletotrichum gloeosporioides, fungal pectinase, polygalacturonsase, pectin estease, polyacrylamide gel electrophoresis