ASSOCIATION OF A B.L.O. TO MARGINAL CHLOROSIS: A NEW DISEASE OF STRAWBERRY
Electron microscopy observation revealed the presence of Bacteria Like Organism (B.L.O.) in the phloem of affected strawberry plants. Moreover DAPI staining carried out on the same samples resulted in fluorescent staining of DNA in the phloem. For these reasons, DAPL staining can be used to investigate the presence of B.L.O. in large numbers of symptomatic plants. Moreover the DAPI test has to be used carefully as it is not specific of the B.L.O. and can also detect other MLO pathogens in strawberry. B.L.O. transmission from strawberry to Catharanthus roseus by dodder is underway. As the insert vector(s) of the disease is unknown, control of the disease is done by eradication of infested plants only in nurseries.