GENETIC MAPPING OF THE DIPLOID STRAWBERRY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS
In order to circumvent the genetic complications inherent in the octoploid genomic structure of the commercial strawberry, Fragaria x ananassa, we have begun to construct a strawberry genetic map at the diploid level, using crosses within Fragaria vesca. Having previously identified a close linkage between the isozyme SKDII (shikimate dehydrogenase) and a locus governing red versus yellow fruit color in a cross between diploid cultivars ‘Yellow Wonder’ and ‘Baron Solemacher’, we are not focussing on a wider cross between ‘Yellow Wonder’ and a highly heterozygous wild accession. Segregation of isozymes and RAPD markers in the F1 generation of this cross has resulted in identification of four linkage groups, the largest of which contains ten markers. Preliminary mapping data from the F2 generation of this cross, which is segregating for a variety of morphological/physiological traits, will be reported.