TRANSIENT GENE EXPRESSION IN STRAWBERRY PROTOPLASTS AND THE RECOVERY OF TRANSGENIC PLANTS

M. Nyman, A. Wallin
A transient B-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10 μg/ml hygromycin produced shoots which exhibited GUS-activity. The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis.
Nyman, M. and Wallin, A. (1993). TRANSIENT GENE EXPRESSION IN STRAWBERRY PROTOPLASTS AND THE RECOVERY OF TRANSGENIC PLANTS. Acta Hortic. 348, 442-442
DOI: 10.17660/ActaHortic.1993.348.90
https://doi.org/10.17660/ActaHortic.1993.348.90

Acta Horticulturae