M. Nyman, A. Wallin
Regeneration of plants can be associated with genetic instability such as polyploidization, DNA-amplification, deletions and changes in the metylation pattern. This induced variation is often referred to as somaclonal variation. We analyzed protoplast-derived plants from cultivated strawberry (Fragaria × ananassa) and found that 27 out of 51 plants were octoploid, 15 hexadecaploid (16x), 8 mixaploid and one diplodecaploid (12x).

Chromosome doubled plants were clearly discernible from the normal, octoploid plants in that they has thicker and less numerous leaves, shorter and thicker petioles and larger flowers. Most of the mixaploid plants were stunted and had wrinkled leaves and some of them did not survive in the field. Variation was also found among the octoploid plants in characteristics such as date of flowering, runner production and length of the petioles. Interestingly there was also a large variation in size, colour and shape among berries from different protoclones. Preliminary experiments indicate that these variations cannot be detected on DNA level using DNA-fingerprinting.

Nyman, M. and Wallin, A. (1993). SOMACLONAL VARIATION IN PROTOPLAST-DERIVED STRAWBERRY PLANTS. Acta Hortic. 348, 443-443
DOI: 10.17660/ActaHortic.1993.348.91

Acta Horticulturae