SELECTION OF POLLINATORS FOR TOMBUL HAZELNUT CULTIVAR
Sivri, Fosa, Kalinkara, Mincane and Palaz cultivars have been used as pollinators. The Sivri cultivar was taken as a control cultivar, and Tombul was self pollinated. The pollen germination characteristics of pollinator cultivars were observed in sucrose solutions of 15%, 20% and 25% respectively, using the hanging drop method and in 14 replicas. The counts were taken from the samples which were left in the incubator for 12 hours at 20 C.
In the determination of the level of sexual incompatibility the development of pollen tubes of the pollinator cultivars in the styles were detected using fluorescent microscopy. 1, 3, 6, 9 and 12 days after pollination the samples were fixed using FAA and then were stained with aniline blue and the observations were made by squashing. The counts were taken by dividing the style into three equal sections and taking separate counts in each one of these sections, while the results of the section that was nearest to the ovarium were effectively taken into consideration.
The highest rate of pollen germination was observed at Fosa cultivar (62.8%) and this was followed by Mincane (45.1%), Palaz (37.5%), Kalinkara (36.4%) and Sivri (31.5%) cultivars respectively. Although varying according to the cultivars, the pollen germination was mostly observed at sucrose concentrations of 20% and 25%. It has been determined that the pollens not only enter from the stigma but also from the sides of the style and there they create the pollen tube, in nuts.
Quite distinct levels of pollen tubes have been found to be existing in the deeper parts of the style of Fosa, Mincane, Palaz and Sivri cultivars, one day after pollination. On the sixth day, a significant increase in the number of pollen tubes in this region is seen, almost in all samples.