EFFECTS OF ROTATION RATE IN ORBITAL SHAKING CULTURE ON EMBRYOID FORMATION OF GARLIC
The effects of rotation rate in orbital shaking culture were investigated using callus derived from shoot tip of garlic, Allium sativum L.. Callus tissue was cultured in Murashige and Skoog (1962) liquid medium which contained 0, 1, and 2 ppm of BA and NAA in various combinations. The rotation rate for the first experiment on culture was 70 rpm (63 days) and the rate was changed to 120 rpm (131 days). The rotations in the second and third experiments on culture were 120 rpm (116 days) and 150 rpm (81 days), respectively. Gall-like bodies (GLB) or ball-like bodies (BLB) were formed and subcultured. Callus tissue only proliferated at 70 rpm. After changing the rotation rate from 70 rpm to 120 rpm, GLBs or BLBs were formed. At rates of 120 rpm and 150 rpm, the formation of GLB and BLB were promoted. At 150 rpm, BLB became separated from the callus tissue and then became embryoid structures. When embryoid tissue was observed by scanning electron microscope, epidermal tissue and vascular bundles had differentiated. When GLB was subcultured on agar medium, it proliferated only callus tissue. However, when BLB was subcultured on medium which contained 1 or 2 ppm of BA and NAA, many shoots were formed.
Fujime, Y., Ono, M.M. and Kudou, R. (1994). EFFECTS OF ROTATION RATE IN ORBITAL SHAKING CULTURE ON EMBRYOID FORMATION OF GARLIC. Acta Hortic. 358, 199-204