HISTOLOGICAL ANALYSIS OF RHODODENDRON LEAVES DURING ACCLIMATIZATION OF IN VITRO PLANTS
Acclimatization and transfer of micropropagated woody ornamentals can be regarded as a difficult culture stage. Histological investigations with Rhododendron 'Pink Pearl' show differences in anatomy of in vitro and ex vitro leaves. Leaves of two year old plants were thicker and are characterized by a multilayered epidermis and palisade tissue and by the presence of a cuticle. Small respiration cavities and a poorly structured mesophyll have been observed with in vitro material. Leaves of the adaptation phase were intermediate between in vitro and container-grown leaves. Acclimatization at low relative humidity in comparison to high relative humidity results in an increase of leaf thickness, stomatal density, and an improved differentiation of the palisade layer and the spongy mesophyll.
Waldenmaier, S. (1994). HISTOLOGICAL ANALYSIS OF RHODODENDRON LEAVES DURING ACCLIMATIZATION OF IN VITRO PLANTS. Acta Hortic. 364, 53-60