TISSUE CULTURE AS A TOOL IN STUDIES OF HOST-VIRUS INTERACTIONS
TMV infection reduces the viability of tomato callus cells from a susceptible but not a resistant cultivar (Murakishi, 1965). Similarly, replication of virus was highest in susceptible and lowest in resistant tobacco callus (Beachy and Murakishi, 1970).
Our primary objective was to develop tissue culture as a tool to study virus replication. Thus, factors which affected the efficiency of infection and virus concentration were of paramount importance to use.