DISTRIBUTION OF APPLE CHLOROTIC LEAFSPOT VIRUS IN APPLE SHOOTS CULTIVATED IN VITRO
An immuno tissue-printing protocol has been worked out for the immunological detection of apple chlorotic leaf spot virus (ACLSV) coat protein within in vitro shoots of Malus.
Different apple varieties showed a typical distribution pattern for ACLSV in vitro. Accumulation of ACLSV was highest in the basal part of the stem and decreased towards the apex of the shoots.
ACLSV reached its maximum amount at the enlarged bases of shoots, where it was unevenly distributed, as tissue was less regularily organized.
In cross sections of stem tissue virus coat protein was found within the epidermis, the cortical parenchyma and within the vascular bundles.
Within pith tissue virus protein was only detected in adjacent parts of the enlarged bases when shoots were heavily infected.
The degree of infection not only influenced the localisation of virus within a cross section, but also its spread into the meristem.
Meristems of poorly infected shoots did not contain any visible trays of virus.
Knapp, E., Hanzer, V., Weiss, H., da Câmara Machado, A., Wang, Q., Weiss, B., Katinger, H. and Laimer da Câmara Machado, M. (1995). DISTRIBUTION OF APPLE CHLOROTIC LEAFSPOT VIRUS IN APPLE SHOOTS CULTIVATED IN VITRO. Acta Hortic. 386, 187-194
DOI: 10.17660/ActaHortic.1995.386.24
https://doi.org/10.17660/ActaHortic.1995.386.24
DOI: 10.17660/ActaHortic.1995.386.24
https://doi.org/10.17660/ActaHortic.1995.386.24
386_24
187-194
- Division Tropical and Subtropical Fruit and Nuts
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Protected Cultivation and Soilless Culture
- Division Postharvest and Quality Assurance
- Division Temperate Tree Fruits
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems