IN VITRO PROPAGATION OF BULB SCALE SECTION OF SEVERAL KOREAN NATIVE LILIES
The effects of light condition for culture and sucrose concentrations, pH values and growth regulators on Murashige and Skoog basal media and conditions of explants on bulblet formation of several Korean native lilies by scale culture were investigated. Rate of bulblet formation and numbers of originated bulblets per scale etc. were much better at light environment than at dark environment. 6 – 8 % sucrose added media were most effective in bulblet formation of scales of Lilium concolor var. partheneion, which is one of Korean native lilies. pH of medium also highly affected regeneration of bulblet. The best result was obtained at pH 5.0 – 6.5. Regeneration ability of bulblets by attached positions of scales was varied according to Lilium species. In L. concolor var. partheneion, the central parts of bulbs, which are remaining part of bulbs after removing of outer and inner scales showed higher bulblet development rate but formed fewer numbers of bulblets per explant than outer and inner scales. The numbers of formed bulblets was most on culture of outer scales. Scales were cut into three parts of distal, central basal parts to culture. The best result was obtained in culture of basal parts. But with treatment of NAA at 0.01 ppm with BA at 0.1 ppm, bulblets and organ formation were much enhanced in distal parts. Those results were much better than in basal parts. Effectiveness of growth regulators on bulblet development was also varied according to species. High concentrations (above 1.0 ppm) of NAA without/with BA greatly inhibited bulblet and other organ forming in all three species of lilies used in this experiment, but low concentration of NAA at 0.01 ppm and/or BA at 0.01–0.1 ppm enhanced bulblet formation. In L. amabile and L. callosum, supplementation with NAA at 1.0–10.0 ppm induced very high rate of callus formation.
Jeong, J.H. (1996). IN VITRO PROPAGATION OF BULB SCALE SECTION OF SEVERAL KOREAN NATIVE LILIES. Acta Hortic. 414, 269-276