PRODUCTION AND IDENTIFICATION OF INTERSPECIFIC HYBRIDS OF LILIUM LONGIFLORUM AND L. x ELEGANS
Lilium longiflorum Thunb. 'Nellie White' and a selection of L. x elegans Thunb. (hybrid 'D') were used to produce interspecific hybrids by cut style pollination, separated carpel and ovule culture. Separated carpels were cultured on a Murashige and Skoog (MS) medium supplemented with NAA at 0, 0.25, 0.5, and 1.0 mg/L and kinetin at 0, 1.5, 3, and 6 mg/L in all possible 16 combinations. Individual ovules that were enlarged and swollen were then removed and cultured in a MS medium supplemented with NAA and/or kinetin. A total of 303 plantlets were obtained from 41 ovules. The number of ovules that regenerated plantlets was not significantly influenced by NAA, kinetin or their combination. However, the most plantlets were obtained on a medium supplemented with 0.5 mg/L NAA plus 3 mg/L kinetin. Interspecific hybrids were identified by karyotype, carbohydrate, and flavonol analyses, the last methods being able to be employed during the early growth stage of hybrids.
Roh, Mark S., Griesbach, Robert J., Lawson, Roger H. and Gross, K. C. (1996). PRODUCTION AND IDENTIFICATION OF INTERSPECIFIC HYBRIDS OF LILIUM LONGIFLORUM AND L. x ELEGANS. Acta Hortic. 414, 93-100