S. Gannoun, S.M. Lionakis, D. Gerasopoulos
Browning of Pistacia terebinthus explants in vitro - taken from 2, 4 and 29-year-old plants was successfully controlled by adding in the medium 100 mg/l ascorbic acid and by immersing the explants prior to culture for 30 minutes in a aqueous solution containing 10g/l polyvinylpyrrolidone (PVP). However, the browning was not controlled when explants were taken from 8 and 26 years old Pistacia vera plants.

Explants from 2 and 4-year-old P. terebinthus plants showed significantly higher proliferation rate in vitro than explants from 1 year old plants or 29-year-old rejuvenated plants. Single node shoot segment explants from 29 years old rejuvenated P. terevinthus plants produced in vitro significantly more shoots than shoot tip explants from 1-year-old plants. Etiolation pretreatement of plant material taken from 1, 2 and 4-year-old P. terebinthus plants did not affect significantly shoot proliferation in vitro. High shoot proliferation was achieved from explants taken from 1-month-old P. vera seedlings, while internal contamination was a serious problem for the establishment of explants particularly for those taken from adult, 8 and 26-year-old, P. vera plants.

Gannoun, S., Lionakis, S.M. and Gerasopoulos, D. (1995). ASPECTS OF IN VITRO CULTURE OF PISTACIA TEREBINTHUS AND PISTACIA VERA. Acta Hortic. 419, 201-206
DOI: 10.17660/ActaHortic.1995.419.32
P. terebinthus, P. vera, micropropagation, in vitro culture, browning, etiolation, rejuvenation

Acta Horticulturae