ROOTING IMPROVEMENT OF PISTACIA VERA L. CV. MATEUR BY IN VITRO CULTURE OF APICES AND CUTTINGS

A. Chatibi, M.E. Kchouk, F. Ben Abdallah, H. Zemni, A. Ghorbel
In vitro multiplication of Pistacia vera L. cultivars has been described using young plantlets issued from seeds, the juvenile state of the explant playing a major role in the success of the technique. Although relatively performant when compared to conventional practices, the technique is still far from opening new efficient opportunities for pistachio breeding. Besides, in vitro multiplication of apices and meristems showed that the major problem which prevents the extension of the method layed in the rooting of plantlets. We present hereafter a reliable methodology for in vitro multiplication and rooting of Pistacia vera L. cv. Mateur initiated from apices and cuttings. This was performed by taking meristems on dormant material. Success of rooting depends tightly on the vigor of plantlets during multiplication. Subculturing period was reduced to twenty days whereas necrosis of apices was overcome by the addition fo NAA. Thiamine at 1.0 mg. l–1 in the medium allowed a better rooting. The phenomenon of red coloration of leaves prior to acclimatization might be overturned by addition of KCl and AlCl3.
Chatibi, A., Kchouk, M.E., Ben Abdallah, F., Zemni, H. and Ghorbel, A. (1995). ROOTING IMPROVEMENT OF PISTACIA VERA L. CV. MATEUR BY IN VITRO CULTURE OF APICES AND CUTTINGS. Acta Hortic. 419, 213-220
DOI: 10.17660/ActaHortic.1995.419.34
https://doi.org/10.17660/ActaHortic.1995.419.34

Acta Horticulturae