AN OXYGEN ELECTRODE METHOD TO DETERMINE EFFICACY OF PLANT DERIVED MATERIAL TO INHIBIT LIPID PEROXIDATION
A method is described to assay the efficacy of natural plant materials to inhibit lipid peroxidation. Using an aqueous system employing linoleic acid in an emulsion with AAPH as a free radical initiator steady state rates of oxygen uptake may be established. The rate of oxygen uptake is proportional to the rate of lipid peroxidation. The addition of aqueous solutions of plant extracts, either crude or refined may be observed to inhibit the rate of oxygen uptake if the product is an effective antioxidant. The system is dynamic, inexpensive and can be used by a technician with minimal training time. A permanent record is available using graph paper, or a more sophisticated record can be obtain by interfacing with a data input computer. Calculations are made directly on the graph paper by measuring ratios of run/rise and comparing percentage increase or decrease of reaction rates. The role of iron in biological system is becoming increasingly complex, but more and more is being discovered about both the beneficial and deletorious aspects of iron.13,14,15 The need for safe and effective iron chelators in human health is becoming more apparent.16 This method is useful for investigators to screen natural product for both antioxidant and chelating activity.
Pugliese, Peter T., Leisawitz, S. and Richardson, N. (1996). AN OXYGEN ELECTRODE METHOD TO DETERMINE EFFICACY OF PLANT DERIVED MATERIAL TO INHIBIT LIPID PEROXIDATION. Acta Hortic. 426, 243-254