IN VITRO DEVELOPMENTAL RESPONSE OF ANISE TO GROWTH REGULATORS AND ESTABLISHMENT OF A CLONAL PROPAGATION SYSTEM
Many products derived from edible and medicinal plants are being investigated for their antimicrobial and cancer chemopreventive potential. The alkyl benzene anethole, derived from anise (Pimpinella anisum) exhibits antimicrobial properties. Anise is a cross pollinating species and is genetically heterogeneous. Investigation into the molecular and physiological regulation of the production of anethole requires the development of genetically uniform clonal lines. To optimize the in vitro development of anise clonal lines, root explants were cultured on Murashige and Skoog (MS) media supplemented with 4 different growth regulators. The synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) induced somatic embryos from callus. Napthalenacetic acid (NAA), also a synthetic auxin, induced prolific root formation. The synthetic cytokinins 6-benzylaminopurine (BAP) and thidiazuron (TDZ) induced adventitious shoot formation. The 2,4-D induced somatic, embryogenesis system followed by plant regeneration was the best system for the establishment of clonal lines of anise.
Bela, John S. and Shetty, K. (1996). IN VITRO DEVELOPMENTAL RESPONSE OF ANISE TO GROWTH REGULATORS AND ESTABLISHMENT OF A CLONAL PROPAGATION SYSTEM. Acta Hortic. 426, 483-488