SOMATIC EMBRYOGENESIS AND IN VITRO SECONDARY METABOLITE PRODUCTION FROM COMMON MALLOW (MALVA SYSLVESTRIS L.) COLLECTED IN GREECE
The secondary metabolite production from mallow tissues cultured on 1.8 μM NAA and 1.8 μM BA was investigated over a culture period of four weeks. Substances were extracted with warm water and partitioned against ethyl acetate. The constituents of the concentrated extracts were separated by thin-layer chromatography (9.5 toluene: 0.5 ethyl acetate (v/v)) and their total concentration was determined spectrophotometrically at 257 nm. A total of at least seven constituents were detected in tissues cultured in vitro, while only one constituent could be detected in stems received from plants growing in vivo. Differences were observed between tissues cultured under different PPFDs. Neither of the detected substances was psoralen, bergapten or 8-methoxypsoralen, which were co-analysed as standard furanocoumarines.
DOI: 10.17660/ActaHortic.1998.457.21
https://doi.org/10.17660/ActaHortic.1998.457.21