ISOLATION AND CHARACTERIZATION OF ERWINIA AMYLOVORA FROM AUSTRALIAN WOOD SAMPLES
In three cases, we obtained positive PCR signals with three primer pairs from plant material from the Royal Botanic Gardens (RBG) in Melbourne and, in another case, from a soft agar culture which was prepared in Australia from a wood sample of the same origin. The isolation of the pathogen was achieved in three of four PCR-positive samples. Once by plating, and twice by inoculation of immature pears. The isolated strains were further confirmed as E. amylovora by tests on selective agar plates, lectin staining, PFGE and sequence analysis of the intergenic transcribed rRNA spacer region.
Strains from samples of the Melbourne RBG isolated in Germany or New Zealand revealed the same PFGE pattern. A difference was found for the length of the amplified fragment with primers from E. amylovora plasmid pEA29. The fire blight pathogen was not detected from any samples obtained from plant material of the Botanic Gardens in Adelaide.
DOI: 10.17660/ActaHortic.1999.489.6
https://doi.org/10.17660/ActaHortic.1999.489.6
- Division Temperate Tree Fruits
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems
- Division Protected Cultivation and Soilless Culture
- Division Postharvest and Quality Assurance
- Division Tropical and Subtropical Fruit and Nuts