DEVELOPMENT OF PRE-ACCLIMATIZED BANANA PLANTLETS OBTAINED FROM TISSUE CULTURE

S. Silva, R.P. Oliveira, A.P. de Matos, S. Silva Neto, A.G. Prazeres, C.A.P. Oliveira
The objective of this study was to evaluate the development of pre-acclimatized banana plantlets cv. Pioneira (Musa sp., group AAAB) obtained from tissue culture. Seedlings were multiplied in five subcultures (30 days each), on MS medium supplemented with 5 mg.L-1 BA (benzylaminopurine), solidified with agar gel, pH 5.8, under 20 hours photoperiod, 2,000 lux of light intensity and temperature of 25±2°C. The in vitro rooting was performed on MS medium. Plantlets were pre-acclimatized for 30 days in a greenhouse, under controlled conditions of temperature and irrigation. These plantlets, 4.8±1.5 cm high, were then transferred to plastic bags containing organic substrate. Growth and development of the plantlets were evaluated under several conditions: greenhouse, humid chamber in greenhouse, screenhouse, humid chamber in screenhouse, under tree canopy, humid chamber under tree canopy and in-field conditions. A completely randomized design was used with five replications, each replicate containing 10 plantlets. Evaluations of plantlet development were performed at 60 and 120 days after transplanting. All treatments showed 100% plantlet growth, except for the direct field transplanting (39.7%). The plantlet development was similar in greenhouse, screenhouse, and under tree canopy conditions. The proposed system shows feasibility for use in long-distance distribution of micropropagated plantlets.
Silva, S., Oliveira, R.P., de Matos, A.P., Silva Neto, S., Prazeres, A.G. and Oliveira, C.A.P. (1998). DEVELOPMENT OF PRE-ACCLIMATIZED BANANA PLANTLETS OBTAINED FROM TISSUE CULTURE. Acta Hortic. 490, 195-200
DOI: 10.17660/ActaHortic.1998.490.19
https://doi.org/10.17660/ActaHortic.1998.490.19
Musa sp., benzylaminopurine, plantlet production, micropropagation
English

Acta Horticulturae