DEVELOPMENT OF PRE-ACCLIMATIZED BANANA PLANTLETS OBTAINED FROM TISSUE CULTURE
The objective of this study was to evaluate the development of pre-acclimatized banana plantlets cv.
Pioneira (Musa sp., group AAAB) obtained from tissue culture.
Seedlings were multiplied in five subcultures (30 days each), on MS medium supplemented with 5 mg.L-1 BA (benzylaminopurine), solidified with agar gel, pH 5.8, under 20 hours photoperiod, 2,000 lux of light intensity and temperature of 25±2°C. The in vitro rooting was performed on MS medium.
Plantlets were pre-acclimatized for 30 days in a greenhouse, under controlled conditions of temperature and irrigation.
These plantlets, 4.8±1.5 cm high, were then transferred to plastic bags containing organic substrate.
Growth and development of the plantlets were evaluated under several conditions: greenhouse, humid chamber in greenhouse, screenhouse, humid chamber in screenhouse, under tree canopy, humid chamber under tree canopy and in-field conditions.
A completely randomized design was used with five replications, each replicate containing 10 plantlets.
Evaluations of plantlet development were performed at 60 and 120 days after transplanting.
All treatments showed 100% plantlet growth, except for the direct field transplanting (39.7%). The plantlet development was similar in greenhouse, screenhouse, and under tree canopy conditions.
The proposed system shows feasibility for use in long-distance distribution of micropropagated plantlets.
Silva, S., Oliveira, R.P., de Matos, A.P., Silva Neto, S., Prazeres, A.G. and Oliveira, C.A.P. (1998). DEVELOPMENT OF PRE-ACCLIMATIZED BANANA PLANTLETS OBTAINED FROM TISSUE CULTURE. Acta Hortic. 490, 195-200
DOI: 10.17660/ActaHortic.1998.490.19
https://doi.org/10.17660/ActaHortic.1998.490.19
DOI: 10.17660/ActaHortic.1998.490.19
https://doi.org/10.17660/ActaHortic.1998.490.19
Musa sp., benzylaminopurine, plantlet production, micropropagation
English
490_19
195-200