BACTERIAL CONTAMINATION IN MUSA SHOOT TIP CULTURES
Culture of 1mm sized meristem tips, isolated from contaminated in vitro cultures, proves to be effective for the elimination of bacteria but risks losing explants (33%) due to establishment failure. However, up to 100% of the growing meristem tips tested negative for bacteria.
In another experiment a range of antibacterial agents i.e. rifampicin, tetracyclin, streptomycin, trimethoprim, penicillin and bacitracin were tested for their ability to control Bacillus pumilis in banana shoot tips. Their phytotoxicity was also examined. Treatment of shoot tips with rifampicin at 100mg.l-1 during 1 month resulted in 100% bacteria-free explants without any phytotoxicity.