EVALUATION OF THREE DISINFECTION PROTOCOLS AND THREE PROTOCOLS FOR THE USE OF ANTI-OXIDANTS IN IN VITRO CULTIVATION OF CHERIMOYA (ANNONA CHERIMOLA MILL.) AND THE QUANTITATIVE DETERMINATION OF BRANCH PHENOLIC CONTENT
The first trial, carried out monthly from June to January, did not show any effect of disinfectant or of the part of the branch used (apical, medium or basal). On the contrary, there was a phenological (seasonal) effect. For phenological reasons, the second trial was only carried out in January. No positive effect was obtained with either of the evaluated oxidants (PVP-6755, ascorbic acid + citric acid, and DIECA). The results obtained in the third trial showed that the lowest phenolic levels for all branch sections are found in June, whereas the highest occur in August and September when vegetative growth of cherimoya decreases and lignification increases.