CLONING AND CHARACTERIZATION OF A PUTATIVE ENDOPOLYGALACTURONASE CDNA FROM RIPENING MANGO (MANGIFERA INDICA LINN CV. NAM DOK MAI)
A cDNA for mango PG was made in order to study the expression of its ripening specific gene. Purified mRNA from ripening mango was used as a template for RT-PCR. Endo and exo-PG sequence from various sources obtained from EMBL data base were aligned. Conserved regions of the sequence were identified and used to design primer for PCR. PCR product were fractionated on an agarose gel and bands with appropriate sizes were eluted and ligated into a plasmid vector. One of the cDNA isolated had a size of 811 bp and encoded a 161 amino acids open reading frames, the deduced sequence of which was related by sequence similarly to plant PGs. A near match was to the endo-PG of kiwi fruit (acession No. P35336) which was 51% similar at the identity level and 67% similar when conserved substitution were allowed. This PG cDNA was used as a probe for hybridisation analysis of fruit RNA preparations.