IN VITRO MORTALITY OF EGGS AND THIRD INSTAR LARVAE OF ANASTREPHA LUDENS AND A. OBLIQUA WITH INSECTICIDAL ATMOSPHERES AT HIGH TEMPERATURES
The in vitro mortality of eggs and third instar larvae of Anastrepha ludens and A. obliqua was determined in air or in controlled atmosphere (CA) at high temperatures. Atmosphere evaluated were air, 0 kPa O2, 13 kPa O2 + 20 kPa CO2, and 0 kPa O2 + 50 kPa CO2 at 44°C for 160 min. Other treatments evaluated included 0 kPa O2 + 50 kPa O2 at 48°C for 160 and 220 min, and the same atmosphere at 40, 50, 51, 52, 54 and 55°C for 80, 160 and 240 min. Dry air at 44°C for 160 min caused very low mortality, and CA significantly increased the mortality. Higher temperatures caused a more rapid kill. A mortality of 100% was first achieved for the third instar larvae of A. ludens in air at 48°C, and then to third instar larvae of both species in CA at 48°C. A 100% mortality of eggs of A. ludens was achieved first in air and in CA at 51°C, and 100% mortality of eggs of A. obliqua was first achieved in air and in CA at 54°C. A obliqua was slightly tolerant than A. ludens, and eggs were more tolerant than third instar larvae in both species. There was clear synergistic effect of CA at temperatures lower than 50°C, however, at 52 and 54°C CA was slightly less effective than air. We conclude that dry-hot air at ≥ 44°C and 50% RH in CA (0 kPa O2 + 50 kPa CO2), for 160 min or longer, is effective in causing high mortality of eggs and third instar larvae of A. ludens and A. obliqua.
Yahia, Elhadi M. and Ortega-Zaleta, D. (2000). IN VITRO MORTALITY OF EGGS AND THIRD INSTAR LARVAE OF ANASTREPHA LUDENS AND A. OBLIQUA WITH INSECTICIDAL ATMOSPHERES AT HIGH TEMPERATURES. Acta Hortic. 509, 779-786
controlled atmosphere, disinfestation, fruit fly, heat, hot air, insects, larvae, quarantine, postharvest