REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION IN STIPULES OF STRAWBERRY

S. Monticelli, A. Gentile, C. Damiano
Competent tissues for regeneration and transformation were identified in the stipules of micropropagated plants of strawberry, cvs Teodora and Egla. The insertion zone of the petiole with the stipules and the basal part of the leaves, along the wounded margins, produced shoots in presence of the BAP. The same sites were susceptible to infection and transformation by Agrobacterium tumefaciens carrying uid-a-int (GUS) and nptII genes. Regeneration performance of stipules coming from plants cultivated in different mineral and hormonal conditions was evaluated. Different infection and kanamycin selection strategies were adopted. A low salt concentration and the absence of hormones in the multiplication medium inhibited regenerations from stipules. A delayed kanamycin selection allowed us to obtain transgenic clones. Further regenerated shoots were obtained from stipules of the transgenic clones. All of them were transgenic. The transformed plants were rooted under kanamycin selection and transferred to the greenhouse for the evaluation of the gene stability and of the expression in different organs. GUS reaction was detected in leaves, sepals, petals, receptacle, anthers. Molecular analysis was carried out by PCR on plant material coming from in vitro and in vivo to confirm gene integration. New experiments were designed to introduce genes for fungal disease resistance.
Monticelli, S., Gentile, A. and Damiano, C. (2002). REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION IN STIPULES OF STRAWBERRY. Acta Hortic. 567, 105-107
DOI: 10.17660/ActaHortic.2002.567.13
https://doi.org/10.17660/ActaHortic.2002.567.13
Fragaria x ananassa, fungal disease resistance, gene stability, GUS expression, kanamycin resistance, PCR
English

Acta Horticulturae