MICROPROPAGATION OF RED PITAYA (HYLOCEREOUS UNDATUS)
Red pitaya, Hylocereous undatus, is a member of cactaceae family that is cultivated for fruit production.
Although conventional methods of propagation of cacti are satisfactory, a protocol for micropropagation has been developed to allow rapid release of improved clones or genotypes. H. undatus does not need plant growth regulators for shoot and root formation and their subsequent growth.
Shoots and roots can be induced in a plant growth regulator-free medium irrespective of the size of the explant.
However, the addition of 5 μM 2ip to MS medium induced 100% of stem segments with intact areols to produce shoots and reduced the time to shoot initiation.
Stem segments with intact shoot apices exhibited strong apical dominance and this effect could not be removed by addition of 5 μM 2iP to the medium.
Stem segments with one whorl of areols produced more shoots per explant than those with 2 or 3 whorls.
Increasing the density of stem explants in a culture flask decreased the percentage of areols that produced shoots however, increased the number of shoots per flask.
Exposure of shoots to 10 μM IBA did not significantly affect rooting percentage or time to root initiation but did affect root length.
Drew, R.A. and Azimi, M. (2002). MICROPROPAGATION OF RED PITAYA (HYLOCEREOUS UNDATUS). Acta Hortic. 575, 93-98
DOI: 10.17660/ActaHortic.2002.575.7
https://doi.org/10.17660/ActaHortic.2002.575.7
DOI: 10.17660/ActaHortic.2002.575.7
https://doi.org/10.17660/ActaHortic.2002.575.7
Cactaceae, cactus, tissue culture, propagation
English
575_7
93-98
- Working Group Horticultural Biotechnology and Breeding
- Working Group Jackfruit and other Moraceae
- Division Plant Genetic Resources and Biotechnology
- Division Horticulture for Development
- Division Tropical and Subtropical Fruit and Nuts
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems