EVALUATION OF NATURAL DIVERSITY AMONG ERWINIA AMYLOVORA ISOLATES ON THE BASIS OF TOTAL CELLULAR PROTEIN AND FATTY ACID PATTERNS
Erwinia amylovora strains, isolated from various host plants growing in distant regions of Poland, were analysed for total cellular protein and fatty acid patterns. The protein profiles obtained after SDS-PAGE revealed high similarity among the studied strains. Only in-depth densitometrical and statistical analyses proved the presence of discreet differences between the isolates. Although fatty acid profiles were also highly comparable, the variations between the isolates were more clearly demonstrated. The predominant protein bands were 64.6, 48.6, 45.2 and 38.3 kDa, whereas hexadecanoic (16:0), methylenehexadecanoic (17:0cyc) and octadecanoic (18:1 7cis) acids comprised the main fatty acids in E. amylovora cells. Both these techniques provided a useful adjunct for a better understanding of the natural diversity observed among various isolates of the same species. Electrophoresis and gas chromatography were found to be convenient and more direct procedures for identification of individual strains of E. amylovora. This experimental approach is also proving to be a powerful, and at the same time a relatively fast and cheap tool for investigating the ecology, epidemiology and spread of particular strains in the environment. Moreover, such analysis might successfully complement other techniques applied in modern molecular plant pathology.
Zarnowski, R., Lewicka, T. and Ellis, R.J. (2002). EVALUATION OF NATURAL DIVERSITY AMONG ERWINIA AMYLOVORA ISOLATES ON THE BASIS OF TOTAL CELLULAR PROTEIN AND FATTY ACID PATTERNS. Acta Hortic. 590, 185-192
fire blight, SDS-PAGE, electrophoresis, identification, pathogen monitoring, pathogen spreading