STANDARDIZATION OF DIAGNOSTIC PROTOCOLS (DIAGPRO) FOR ERWINIA AMYLOVORA IN THE EUROPEAN UNION
Different reagents and methods have been reviewed, selected, improved standardized and validated for detection and diagnosis of Erwinia amylovora. The validated methods and protocols will be published on an Internet web-site for comments and will be included as the European Union official scheme for the detection and identification of the bacterium. A combination of two different methods based on serology and PCR in addition to isolation followed by a pathogenicity test, will be required to officially confirm the E. amylovora presence. The detection of the bacterium in symptomless plant material must be assayed after a validated enrichment step.
López, M.M., Gorris, M.T., Llop, P., Peñalver, J. and Cambra, M. (2002). STANDARDIZATION OF DIAGNOSTIC PROTOCOLS (DIAGPRO) FOR ERWINIA AMYLOVORA IN THE EUROPEAN UNION. Acta Hortic. 590, 69-72
Fire blight, extraction, tissue print ELISA, enrichment DASI ELISA, PCR, immunofluorescence, monoclonal antibodies, polyclonal antibodies.