THE OCCURRENCE AND MOLECULAR CHARACTERISATION OF PNRSV ISOLATES IN TURKEY
Totally 486 plant materials of sour cherry, sweet cherry, peach, nectarine, apricot and plum trees originating from different stone fruit growing regions of Turkey was investigated for the occurrence of PNRSV in fruit orchards and nurseries.
DAS-ELISA and RT-PCR procedures were applied to all samples in order to determine the present viruses. 51 samples were estimated as infected by PNRSV testing by RT-PCR, while DAS-ELISA detected the virus only in 31 samples.
PCR-RFLP analyses were performed by restriction endonucleases and created a phylogenetic tree. 20 Turkish isolates had no identical pattern with PE-5 group of PNRSV isolates.
Only Bursa21 isolate was determined as a member of PV-32 group.
Our PNRSV isolates obtained from different geographic locations did not reveal any association on grouping based on the results of PCR-RFLP.
Ulubas, C. and Ertunc, F. (2004). THE OCCURRENCE AND MOLECULAR CHARACTERISATION OF PNRSV ISOLATES IN TURKEY. Acta Hortic. 657, 115-120
DOI: 10.17660/ActaHortic.2004.657.14
https://doi.org/10.17660/ActaHortic.2004.657.14
DOI: 10.17660/ActaHortic.2004.657.14
https://doi.org/10.17660/ActaHortic.2004.657.14
PNRSV, RT-PCR, PCR-RFLP
English
657_14
115-120
- Division Tropical and Subtropical Fruit and Nuts
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Protected Cultivation and Soilless Culture
- Division Postharvest and Quality Assurance
- Division Temperate Tree Fruits
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems