PHYTOPLASMA DISEASES OF STONE FRUIT TREES IN POLAND
Symptoms typical for phytoplasma diseases were observed recently on peach, apricot nectarine, and sweet cherry trees growing in experimental orchards in Poland.
PCR/RFLP analysis were carried out to identify the causal agents of diseases.
Nucleic acid was extracted from phloem tissue.
The target DNA were amplified by nested PCR with universal primers R16F2n/R2 and also specific primer pairs R16(I)F1/R1, R16(III)F2/R1, R16(V)F1/R1, and R16(X)F1/R1 followed by P1/P7 primers.
Using R16F2n/R2 and R16(V)F1/R1 primer pairs, all samples of DNA extracted from infected trees yielded a nested PCR products of the appropriate size.
No PCR products were obtained from healthy trees.
RFLP analyses of the R16F2n/R2 amplified fragments revealed that the phytoplasma isolated from the affected peach, nectarine, apricot, and sweet cherry trees is closely related to the elm yellows group (16SrV).
Cieślińska, M., Morgaś, H. and Jakubowski, T. (2004). PHYTOPLASMA DISEASES OF STONE FRUIT TREES IN POLAND. Acta Hortic. 657, 523-526
DOI: 10.17660/ActaHortic.2004.657.85
https://doi.org/10.17660/ActaHortic.2004.657.85
DOI: 10.17660/ActaHortic.2004.657.85
https://doi.org/10.17660/ActaHortic.2004.657.85
sweet cherry, peach, apricot, nectarine, PCR, RFLP
English
657_85
523-526
- Division Tropical and Subtropical Fruit and Nuts
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Protected Cultivation and Soilless Culture
- Division Postharvest and Quality Assurance
- Division Temperate Tree Fruits
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems