AN IN VITRO BIOASSAY TO EVALUATE SWEET CHERRY RESPONSE TO INOCULATION WITH PSEUDOMONAS SYRINGAE PV. SYRINGAE
An excised leaf bioassay from sweet cherry tissue culture plantlets was developed to test for a necrotic response to inoculation with Pseudomonas syringae pv. syringae. The four treatments in this bioassay included a water control, a low virulence 108cfu/ml P. syringae inoculation, and a 106 or a 108cfu/ml mixed pathogenic treatment. Leaves were inoculated and then incubated for 7 days. Evaluation of the necrotic response used a rating scale of 0 to 4 with 0 denoting necrosis surrounding the wound site only and 4 denoting necrosis of the entire leaf. None of the evaluated genotypes exhibited any necrosis when inoculated with the water control, yet a few genotypes exhibited negligible necrosis when inoculated with a low virulence strain, JL2000. Several of the genotypes tested had necrotic responses statistically the same as the susceptible control (Corum) when inoculated with either mixed pathogenic treatment. Weiroot genotypes had a high necrotic rating, Pi-KU and Giessen/GiSelA genotypes had variable ratings, and Mazzard x Mahaleb (MxM) genotypes had very low necrotic ratings.
Roche, M. and Azarenko, A.N. (2005). AN IN VITRO BIOASSAY TO EVALUATE SWEET CHERRY RESPONSE TO INOCULATION WITH PSEUDOMONAS SYRINGAE PV. SYRINGAE. Acta Hortic. 667, 503-508
Bacterial canker, rootstocks, Prunus avium, P. cerasus, P. mahaleb, interspecific Prunus hybrids