THE ESTABLISHMENT AND SCREENING OF HOP GENOMIC AND CDNA LIBRARIES FOR GENES DETERMINING LUPULIN PRODUCTION
An analysis of in vivo expression of chalcone synthase homologues on both RNA and protein levels in different phenotypes indicated the presence of fine regulatory factors involved in determination of lupulin biosynthesis. As the major interest, the genetic determination of expression of hop chalcone synthase CHS_H1 was followed. This true-chs homologue we analyzed previously as a major component having high affinity for biosynthesis of prenylated flavonoids and detectable affinity for precursors of bitter acids. For detailed analysis of chs genes and the cis and trans regulatory factors involved in lupulin production, genomic and expression libraries were established. In the initial screenings as much as 15 chs-specific clones were isolated from the genomic library and analyzed. Approximately two thousand of clones hybridized with the CHS homologues suggesting an enrichment of the cDNA library for chs sequences.
Novak, P., Vrba, L., Matoušek, J., Patzak, J. and Fussy, Z. (2005). THE ESTABLISHMENT AND SCREENING OF HOP GENOMIC AND CDNA LIBRARIES FOR GENES DETERMINING LUPULIN PRODUCTION. Acta Hortic. 668, 81-84
chalcone synthase, CHS, homologues, valerophenone synthase, VPS