THE ELIMINATION OF VIRUSES AND HOP LATENT VIROID FROM HOP (HUMULUS LUPULUS L.) IN POLAND

M. Grudzińska, E. Solarska
Hops in Poland are commonly infected with Hop latent viroid (HLVd) and viruses: Prunus necrotic ringspot virus (PNRSV) and Hop mosaic virus (HpMV). Previous experiments were successful in obtaining virus-free hop plants by heat therapy method but appeared to be inefficient for HLVd elimination. In these studies virus and viroid free hop plants were obtained by meristem-tip culture. The meristem tips were excised from lateral shoot tips of naturally infected plants and placed in vitro. In vitro regeneration ability varied between 0 and 46% of regenerated explants depending upon cultivar, depending upon the time at which meristems tips were obtained. The regenerated plants were tested by ELISA for the presence of viruses and by RT-PCR for the presence of HLVd. Plants with no detectable viruses and HLVd were used for further propagation.
Grudzińska, M. and Solarska, E. (2005). THE ELIMINATION OF VIRUSES AND HOP LATENT VIROID FROM HOP (HUMULUS LUPULUS L.) IN POLAND. Acta Hortic. 668, 149-152
DOI: 10.17660/ActaHortic.2005.668.19
https://doi.org/10.17660/ActaHortic.2005.668.19
virus and viroid elimination, in vitro culture, meristem tip, hop plants, PNRSV, HpMV, HLVd
English

Acta Horticulturae