SOME PROPERTIES OF PLUM POX VIRUS AND ITS NUCLEIC ACID AND PROTEIN COMPONENTS
Antiserum to this strain was prepared and its properties were investigated by different techniques : microprecipitin test, gel double diffusion, electroimmunodiffusion. Immunization and serological tests were carried out using formaldehyde treated antigens.
Nucleic acid was obtained by treating purified virus suspensions with sodium dodecylsulfate in different media. Purified by ultracentrifugation on sucrose gradients or electrophoresis on polyacrylamide gels, plum pox nucleic acid appears to be a single infective molecule with an estimated Mol. wt. of 3.5 x 106.
Coat protein was extracted by heating virus suspension with SDS in the presence of mercaptoethanol and urea. The resulting solution, analysed on polyacrylamide gels, gives three bands as has been observed with some viruses of the Potyvirus group. The estimated Mol. wt. of these three polypeptides are 27,000 ; 29,000 and 43,500.
DOI: 10.17660/ActaHortic.1976.67.22
https://doi.org/10.17660/ActaHortic.1976.67.22
- Division Temperate Tree Fruits
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems
- Division Protected Cultivation and Soilless Culture
- Division Postharvest and Quality Assurance
- Division Tropical and Subtropical Fruit and Nuts