MICROGRAFTS WITH MERISTEMATIC TISSUES, A POSSIBLE TECHNIQUE TO ELIMINATE VIRUSES FROM PRUNUS TREES
Following an anatomical study of Prunus meristematic tips, we decided to consider three size categories of grafts: under 400 μm (meristematic tissues), between 400 and 600 μm (at the limit of differentiated tissues) and above 600 μm, (including differentiated vessels and pith parenchyma). Two micrografting techniques were applie, a side graft on the main shoot of the host and a terminal graft on the cut shoot tip. The micrografts involved light sterilisation of the cut incision but the plants were held under ensterilised conditions. About 3 % of the grafts, on 600 trials, produced a new shoot. All the successful grafts were obtained with the lateral micrograft technique, for P. persica and P. cistena as graft on P. persica GF 305, as rootstock. All the micrografts with tips of less than 400 μm failed.
An antomical study of the developed grafts revealed the manner in which the graft union developed. Moreover, the causes of defects could be explained. They consisted of the necrosis of the explant mainly due to invasion by microorganisms.
Colin, J. and Verhoyen, M. (1976). MICROGRAFTS WITH MERISTEMATIC TISSUES, A POSSIBLE TECHNIQUE TO ELIMINATE VIRUSES FROM PRUNUS TREES. Acta Hortic. 67, 97-102