PHENOLIC COUMPOUNDS AS A FACTOR OF DIFFERENT ROOTING RESPONSE IN DIFFERENT CHESTNUT HYBRID CLONES (CASTANEA CRENATA X CASTANEA SATIVA)

The involvement of different phenolics in rooting process of two in rooting response very different chestnut hybrid clones Marsol and Maraval (Castanea crenata × Castanea sativa) was tested. The experiment was carried out with leafy cuttings of six-year-old in vitro derived stock plants in plastic house under fog system. Different phenolic compounds (sinapic, caffeic, chlorogenic, ellagic, ferulic, p-coumaric acid, rutin, quercitrin and hyperoside) were measured in the root initiation phase (cutting time, one day, four and six days after cutting) in cutting bases and cutting leaves. Cutting leaves of the better rooted clone Maraval contained on average nearly 10-times more chlorogenic acid and nearly 7-times more ellagic acid as for rooting less suitable clone Marsol. Rutin, quercitrin and hyperoside were also present in higher concentrations in cutting leaves of the clone Maraval than in leaves of the clone Marsol. In cutting bases of the clone Marsol the overaccumulation of different hydroxycinnamic acids occurred till the fourth day after being put in the substrate. During the same time in the clone Maraval the previous cited acids significantly decreased. Regarding caffeic and sinapic acids the interaction between clone and type of cuttings (terminal, basal) was noticed. Terminal cuttings of the clone Maraval reacted similar as the Maraval cuttings in general (terminal and basal cuttings together) with decreasing values after being put in the substrate. In contrary, the behaviour of terminal cuttings of the clone Marsol was similar to that in Marsol cuttings in general (terminal and basal cuttings together) or in basal cuttings in both clones with overaccumulation of caffeic and sinapic acid during the first days after cutting.