EVALUATION OF A MAPPING STRATEGY BASED ON SYNTENY: USING THE PRUNUS REFERENCE MAP FOR APRICOT

P. Lambert, L.S. Hagen, J.M. Audergon, P. Arus
The results obtained in the European Prunus mapping project allowed to detect a high level of macro-synteny between the genomes of different Prunus species but apricot. A saturated linkage map with 246 markers (11 isozymes and 235 RFLPs) was constructed in that project using an interspecific F2 progeny between almond and peach. Taking these results into account, we made the initial hypothesis that macro-synteny would also be conserved between apricot (Prunus armeniaca, sub-genus Prunophora, section Armeniaca), peach (P. persica, sub-genus Amygdalus) and almond (P. amygdalus, sub-genus Amygdalus) and considered the almond X peach map as a reference for constructing a map of apricot. The F1 population chosen for mapping was obtained from a cross between P. armeniaca ‘Polonais’ and ’Stark Early Orange’. Segregation blots were made for 152 individuals of this progeny and 5 restriction enzymes. One hundred seventy one RFLP probes utilized for the reference map were chosen to cover the 8 linkage groups. Eighty-three of them were tested on the mapping blots. Thirty-five peach and 7 cherry microsatellites were also tested and 10 peach microsatellites were mapped on the F1 progeny. Separate maps were made for each parent according to a double pseudo-testcross strategy. All markers were distributed in 8 linkage groups in ‘Polonais’ and 9 groups in ‘Stark Early Orange’ where two different groups corresponded to group one in ‘Polonais’. The macro-synteny was well conserved between apricot and the other species and all the markers present in each linkage group were aligned with those of the reference map, except eight markers.
Lambert, P., Hagen, L.S., Audergon, J.M. and Arus, P. (2006). EVALUATION OF A MAPPING STRATEGY BASED ON SYNTENY: USING THE PRUNUS REFERENCE MAP FOR APRICOT. Acta Hortic. 701, 295-300
DOI: 10.17660/ActaHortic.2006.701.44
https://doi.org/10.17660/ActaHortic.2006.701.44
Prunus armeniaca, RFLP, Microsatellites, map comparison, pseudo-testcross
English

Acta Horticulturae