SELECTION OF WOODY INDICATORS AND THE OPTIMUM PLANT MATERIAL AND SAMPLING TIME FOR PHYTOPLASMA ESFY DETECTION
The health of plant material is one of the limiting factors in growing apricot and peach trees in the Czech Republic and Europe. In terms of the EPPO certification scheme, it was necessary to verify the optimal method for phytoplasma ESFY (European stem fruit yellows phytoplasma) detection, regarding the type of plant tissue and the sampling time. The results of PCR detection were compared with visual phytoplasma symptoms. At the same time, we searched among 26 cultivars of stone fruits for the best woody indicators for fast diagnostic detection of phytoplasma ESFY. From the tested indicators, we observed the strongest visual symptoms in the cultivars Tomcot, Leskora, LE-2927 and Bergeron; weak appearance occurred for genotypes Velkopavlovicka, Veecot and M-LE-1. Detection of ESFY carried out with DNA isolated from phloem was higher than for DNA isolated from leaf stalk. The optimum times for taking plant samples for detection of ESFY were June and September; the worst was August. Phloem samples were more exact than samples from leaf stalk. The results were substantiated statistically by the Kruskal-Wallis one-factor analysis of variance and by the method of multiple comparisons for the t-distribution (95% t-interval).
Necas, T. and Krska, B. (2006). SELECTION OF WOODY INDICATORS AND THE OPTIMUM PLANT MATERIAL AND SAMPLING TIME FOR PHYTOPLASMA ESFY DETECTION. Acta Hortic. 717, 101-106
European stone fruit yellows phytoplasma, ESFY, apricot, disease, isolation of DNA, PCR