CLONING AND MAPPING OF RESISTANCE GENE HOMOLOGUES IN APRICOT (PRUNUS ARMENIACA L.)
Genomic DNA sequences, sharing homology with the NBS-LRR (Nucleotide Binding Site - Leucine Rich Repeat) resistance genes, were isolated and cloned from apricot using a PCR approach with degenerate primers designed from conserved regions of the NBS domain. RGA-specific primers were developed from the consensus sequences of each apricot RGA family and were used to develop AFLP-RGA markers by means of an AFLP-modified procedure, where one standard primer is substituted by an RGA-specific primer. Using this method, 27 polymorphic markers, six of which shared homology with the TIR class of the NBS-LRR R-genes, were obtained from 17 different primer combinations. Sixteen of the 27 mapped in an apricot genetic map previously constructed from the self-pollination of the cultivar Lito.
Soriano, J.M., Romero, C., Vilanova, S., LJacer, G. and Badenes, M.L. (2006). CLONING AND MAPPING OF RESISTANCE GENE HOMOLOGUES IN APRICOT (PRUNUS ARMENIACA L.). Acta Hortic. 717, 115-118
NBS-LRR sequences, RGAs, apricot